Ssb2/Nabp1 is dispensable for thymic maturation, male fertility, and DNA repair in mice.
Identifieur interne : 002811 ( Main/Exploration ); précédent : 002810; suivant : 002812Ssb2/Nabp1 is dispensable for thymic maturation, male fertility, and DNA repair in mice.
Auteurs : Didier Boucher [France] ; Therese Vu [France] ; Amanda L. Bain [France] ; Marina Tagliaro-Jahns [France] ; Wei Shi [France] ; Steven W. Lane [France] ; Kum Kum Khanna [Australie]Source :
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology [ 1530-6860 ] ; 2015.
Descripteurs français
- KwdFr :
- Animaux, Fibroblastes (métabolisme), Fibroblastes (physiologie), Fécondité (génétique), Liaison aux protéines (génétique), Mâle, Protéines de liaison à l'ADN (métabolisme), Protéines de transport (métabolisme), Rate (métabolisme), Rate (physiologie), Régulation positive (génétique), Réparation de l'ADN (génétique), Souris, Souris de lignée C57BL, Souris knockout, Thymus (glande) (métabolisme), Thymus (glande) (physiologie).
- MESH :
- génétique : Fécondité, Liaison aux protéines, Régulation positive, Réparation de l'ADN.
- métabolisme : Fibroblastes, Protéines de liaison à l'ADN, Protéines de transport, Rate, Thymus (glande).
- physiologie : Fibroblastes, Rate, Thymus (glande).
- Animaux, Mâle, Souris, Souris de lignée C57BL, Souris knockout.
English descriptors
- KwdEn :
- Animals, Carrier Proteins (metabolism), DNA Repair (genetics), DNA-Binding Proteins (metabolism), Fertility (genetics), Fibroblasts (metabolism), Fibroblasts (physiology), Male, Mice, Mice, Inbred C57BL, Mice, Knockout, Protein Binding (genetics), Spleen (metabolism), Spleen (physiology), Thymus Gland (metabolism), Thymus Gland (physiology), Up-Regulation (genetics).
- MESH :
- chemical , metabolism : Carrier Proteins, DNA-Binding Proteins.
- genetics : DNA Repair, Fertility, Protein Binding, Up-Regulation.
- metabolism : Fibroblasts, Spleen, Thymus Gland.
- physiology : Fibroblasts, Spleen, Thymus Gland.
- Animals, Male, Mice, Mice, Inbred C57BL, Mice, Knockout.
Abstract
SSB1 and SSB2 are newly identified single-stranded (ss) DNA binding proteins that play a crucial role in genome maintenance in humans. We recently generated a knockout mouse model of Ssb1 and revealed its essential role for neonatal survival. Notably, we found compensatory up-regulation of Ssb2 protein levels in multiple tissues of conditional Ssb1(-/-) mice, suggesting functional compensation between these 2 proteins. We report here the first description of Ssb2(-/-) knockout mice. Surprisingly, unlike Ssb1 knockout mice, Ssb2(-/-) mice are viable and fertile and do not exhibit marked phenotypic changes when compared with their Ssb2(+/+) and Ssb2(+/-) littermates. Notably, we did not detect any pathologic changes in the thymus, spleen, or testes, tissues with the most abundant expression of Ssb2. Moreover, Ssb2(-/-) mouse embryonic fibroblasts (MEFs) did not show any sensitivity to DNA-damaging agents, or defects in DNA repair capacity. However, we observed modest up-regulation of Ssb1 levels in Ssb2(-/-) MEFs as well as in Ssb2(-/-) thymus and spleen, suggesting that Ssb1 is likely able to compensate for the loss of Ssb2 in mice. Altogether, our results show that Ssb2 is dispensable for embryogenesis and adult tissue homeostasis, including thymopoiesis, splenic development, male fertility, and DNA repair in mice.
DOI: 10.1096/fj.14-269944
PubMed: 25917330
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 002B17
- to stream PubMed, to step Curation: 002A46
- to stream PubMed, to step Checkpoint: 002A46
- to stream Ncbi, to step Merge: 002507
- to stream Ncbi, to step Curation: 002507
- to stream Ncbi, to step Checkpoint: 002507
- to stream Main, to step Merge: 002811
- to stream Main, to step Curation: 002811
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Ssb2/Nabp1 is dispensable for thymic maturation, male fertility, and DNA repair in mice.</title><author><name sortKey="Boucher, Didier" sort="Boucher, Didier" uniqKey="Boucher D" first="Didier" last="Boucher">Didier Boucher</name><affiliation wicri:level="3"><nlm:affiliation>*Signal Transduction Laboratory, Translational Leukaemia Research, QIMR Berghofer Medical Research Institute, Herston, Queensland, Australia; University of Queensland, Brisbane, Queensland, Australia; Institut National De La Recherche Agronomique, Institut Jean-Pierre Bourgin, Unité Mixte de Recherche 1318, Équipes de Recherche Labellisées Centre National de la Recherche Scientifique 3559, Saclay Plant Sciences, Versailles, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>*Signal Transduction Laboratory, Translational Leukaemia Research, QIMR Berghofer Medical Research Institute, Herston, Queensland, Australia; University of Queensland, Brisbane, Queensland, Australia; Institut National De La Recherche Agronomique, Institut Jean-Pierre Bourgin, Unité Mixte de Recherche 1318, Équipes de Recherche Labellisées Centre National de la Recherche Scientifique 3559, Saclay Plant Sciences, Versailles</wicri:regionArea><placeName><region type="region">Île-de-France</region><region type="old region">Île-de-France</region><settlement type="city">Versailles</settlement></placeName></affiliation></author><author><name sortKey="Vu, Therese" sort="Vu, Therese" uniqKey="Vu T" first="Therese" last="Vu">Therese Vu</name><affiliation wicri:level="3"><nlm:affiliation>*Signal Transduction Laboratory, Translational Leukaemia Research, QIMR Berghofer Medical Research Institute, Herston, Queensland, Australia; University of Queensland, Brisbane, Queensland, Australia; Institut National De La Recherche Agronomique, Institut Jean-Pierre Bourgin, Unité Mixte de Recherche 1318, Équipes de Recherche Labellisées Centre National de la Recherche Scientifique 3559, Saclay Plant Sciences, Versailles, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>*Signal Transduction Laboratory, Translational Leukaemia Research, QIMR Berghofer Medical Research Institute, Herston, Queensland, Australia; University of Queensland, Brisbane, Queensland, Australia; Institut National De La Recherche Agronomique, Institut Jean-Pierre Bourgin, Unité Mixte de Recherche 1318, Équipes de Recherche Labellisées Centre National de la Recherche Scientifique 3559, Saclay Plant Sciences, Versailles</wicri:regionArea><placeName><region type="region">Île-de-France</region><region type="old region">Île-de-France</region><settlement type="city">Versailles</settlement></placeName></affiliation></author><author><name sortKey="Bain, Amanda L" sort="Bain, Amanda L" uniqKey="Bain A" first="Amanda L" last="Bain">Amanda L. Bain</name><affiliation wicri:level="3"><nlm:affiliation>*Signal Transduction Laboratory, Translational Leukaemia Research, QIMR Berghofer Medical Research Institute, Herston, Queensland, Australia; University of Queensland, Brisbane, Queensland, Australia; Institut National De La Recherche Agronomique, Institut Jean-Pierre Bourgin, Unité Mixte de Recherche 1318, Équipes de Recherche Labellisées Centre National de la Recherche Scientifique 3559, Saclay Plant Sciences, Versailles, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>*Signal Transduction Laboratory, Translational Leukaemia Research, QIMR Berghofer Medical Research Institute, Herston, Queensland, Australia; University of Queensland, Brisbane, Queensland, Australia; Institut National De La Recherche Agronomique, Institut Jean-Pierre Bourgin, Unité Mixte de Recherche 1318, Équipes de Recherche Labellisées Centre National de la Recherche Scientifique 3559, Saclay Plant Sciences, Versailles</wicri:regionArea><placeName><region type="region">Île-de-France</region><region type="old region">Île-de-France</region><settlement type="city">Versailles</settlement></placeName></affiliation></author><author><name sortKey="Tagliaro Jahns, Marina" sort="Tagliaro Jahns, Marina" uniqKey="Tagliaro Jahns M" first="Marina" last="Tagliaro-Jahns">Marina Tagliaro-Jahns</name><affiliation wicri:level="3"><nlm:affiliation>*Signal Transduction Laboratory, Translational Leukaemia Research, QIMR Berghofer Medical Research Institute, Herston, Queensland, Australia; University of Queensland, Brisbane, Queensland, Australia; Institut National De La Recherche Agronomique, Institut Jean-Pierre Bourgin, Unité Mixte de Recherche 1318, Équipes de Recherche Labellisées Centre National de la Recherche Scientifique 3559, Saclay Plant Sciences, Versailles, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>*Signal Transduction Laboratory, Translational Leukaemia Research, QIMR Berghofer Medical Research Institute, Herston, Queensland, Australia; University of Queensland, Brisbane, Queensland, Australia; Institut National De La Recherche Agronomique, Institut Jean-Pierre Bourgin, Unité Mixte de Recherche 1318, Équipes de Recherche Labellisées Centre National de la Recherche Scientifique 3559, Saclay Plant Sciences, Versailles</wicri:regionArea><placeName><region type="region">Île-de-France</region><region type="old region">Île-de-France</region><settlement type="city">Versailles</settlement></placeName></affiliation></author><author><name sortKey="Shi, Wei" sort="Shi, Wei" uniqKey="Shi W" first="Wei" last="Shi">Wei Shi</name><affiliation wicri:level="3"><nlm:affiliation>*Signal Transduction Laboratory, Translational Leukaemia Research, QIMR Berghofer Medical Research Institute, Herston, Queensland, Australia; University of Queensland, Brisbane, Queensland, Australia; Institut National De La Recherche Agronomique, Institut Jean-Pierre Bourgin, Unité Mixte de Recherche 1318, Équipes de Recherche Labellisées Centre National de la Recherche Scientifique 3559, Saclay Plant Sciences, Versailles, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>*Signal Transduction Laboratory, Translational Leukaemia Research, QIMR Berghofer Medical Research Institute, Herston, Queensland, Australia; University of Queensland, Brisbane, Queensland, Australia; Institut National De La Recherche Agronomique, Institut Jean-Pierre Bourgin, Unité Mixte de Recherche 1318, Équipes de Recherche Labellisées Centre National de la Recherche Scientifique 3559, Saclay Plant Sciences, Versailles</wicri:regionArea><placeName><region type="region">Île-de-France</region><region type="old region">Île-de-France</region><settlement type="city">Versailles</settlement></placeName></affiliation></author><author><name sortKey="Lane, Steven W" sort="Lane, Steven W" uniqKey="Lane S" first="Steven W" last="Lane">Steven W. Lane</name><affiliation wicri:level="3"><nlm:affiliation>*Signal Transduction Laboratory, Translational Leukaemia Research, QIMR Berghofer Medical Research Institute, Herston, Queensland, Australia; University of Queensland, Brisbane, Queensland, Australia; Institut National De La Recherche Agronomique, Institut Jean-Pierre Bourgin, Unité Mixte de Recherche 1318, Équipes de Recherche Labellisées Centre National de la Recherche Scientifique 3559, Saclay Plant Sciences, Versailles, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>*Signal Transduction Laboratory, Translational Leukaemia Research, QIMR Berghofer Medical Research Institute, Herston, Queensland, Australia; University of Queensland, Brisbane, Queensland, Australia; Institut National De La Recherche Agronomique, Institut Jean-Pierre Bourgin, Unité Mixte de Recherche 1318, Équipes de Recherche Labellisées Centre National de la Recherche Scientifique 3559, Saclay Plant Sciences, Versailles</wicri:regionArea><placeName><region type="region">Île-de-France</region><region type="old region">Île-de-France</region><settlement type="city">Versailles</settlement></placeName></affiliation></author><author><name sortKey="Khanna, Kum Kum" sort="Khanna, Kum Kum" uniqKey="Khanna K" first="Kum Kum" last="Khanna">Kum Kum Khanna</name><affiliation wicri:level="1"><nlm:affiliation>*Signal Transduction Laboratory, Translational Leukaemia Research, QIMR Berghofer Medical Research Institute, Herston, Queensland, Australia; University of Queensland, Brisbane, Queensland, Australia; Institut National De La Recherche Agronomique, Institut Jean-Pierre Bourgin, Unité Mixte de Recherche 1318, Équipes de Recherche Labellisées Centre National de la Recherche Scientifique 3559, Saclay Plant Sciences, Versailles, France kumkum.khanna@qimrberghofer.edu.au.</nlm:affiliation><country wicri:rule="url">Australie</country><wicri:regionArea>*Signal Transduction Laboratory, Translational Leukaemia Research, QIMR Berghofer Medical Research Institute, Herston, Queensland, Australia; University of Queensland, Brisbane, Queensland, Australia; Institut National De La Recherche Agronomique, Institut Jean-Pierre Bourgin, Unité Mixte de Recherche 1318, Équipes de Recherche Labellisées Centre National de la Recherche Scientifique 3559, Saclay Plant Sciences, Versailles</wicri:regionArea><wicri:noRegion>Versailles</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2015">2015</date><idno type="RBID">pubmed:25917330</idno><idno type="pmid">25917330</idno><idno type="doi">10.1096/fj.14-269944</idno><idno type="wicri:Area/PubMed/Corpus">002B17</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002B17</idno><idno type="wicri:Area/PubMed/Curation">002A46</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002A46</idno><idno type="wicri:Area/PubMed/Checkpoint">002A46</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002A46</idno><idno type="wicri:Area/Ncbi/Merge">002507</idno><idno type="wicri:Area/Ncbi/Curation">002507</idno><idno type="wicri:Area/Ncbi/Checkpoint">002507</idno><idno type="wicri:Area/Main/Merge">002811</idno><idno type="wicri:Area/Main/Curation">002811</idno><idno type="wicri:Area/Main/Exploration">002811</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Ssb2/Nabp1 is dispensable for thymic maturation, male fertility, and DNA repair in mice.</title><author><name sortKey="Boucher, Didier" sort="Boucher, Didier" uniqKey="Boucher D" first="Didier" last="Boucher">Didier Boucher</name><affiliation wicri:level="3"><nlm:affiliation>*Signal Transduction Laboratory, Translational Leukaemia Research, QIMR Berghofer Medical Research Institute, Herston, Queensland, Australia; University of Queensland, Brisbane, Queensland, Australia; Institut National De La Recherche Agronomique, Institut Jean-Pierre Bourgin, Unité Mixte de Recherche 1318, Équipes de Recherche Labellisées Centre National de la Recherche Scientifique 3559, Saclay Plant Sciences, Versailles, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>*Signal Transduction Laboratory, Translational Leukaemia Research, QIMR Berghofer Medical Research Institute, Herston, Queensland, Australia; University of Queensland, Brisbane, Queensland, Australia; Institut National De La Recherche Agronomique, Institut Jean-Pierre Bourgin, Unité Mixte de Recherche 1318, Équipes de Recherche Labellisées Centre National de la Recherche Scientifique 3559, Saclay Plant Sciences, Versailles</wicri:regionArea><placeName><region type="region">Île-de-France</region><region type="old region">Île-de-France</region><settlement type="city">Versailles</settlement></placeName></affiliation></author><author><name sortKey="Vu, Therese" sort="Vu, Therese" uniqKey="Vu T" first="Therese" last="Vu">Therese Vu</name><affiliation wicri:level="3"><nlm:affiliation>*Signal Transduction Laboratory, Translational Leukaemia Research, QIMR Berghofer Medical Research Institute, Herston, Queensland, Australia; University of Queensland, Brisbane, Queensland, Australia; Institut National De La Recherche Agronomique, Institut Jean-Pierre Bourgin, Unité Mixte de Recherche 1318, Équipes de Recherche Labellisées Centre National de la Recherche Scientifique 3559, Saclay Plant Sciences, Versailles, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>*Signal Transduction Laboratory, Translational Leukaemia Research, QIMR Berghofer Medical Research Institute, Herston, Queensland, Australia; University of Queensland, Brisbane, Queensland, Australia; Institut National De La Recherche Agronomique, Institut Jean-Pierre Bourgin, Unité Mixte de Recherche 1318, Équipes de Recherche Labellisées Centre National de la Recherche Scientifique 3559, Saclay Plant Sciences, Versailles</wicri:regionArea><placeName><region type="region">Île-de-France</region><region type="old region">Île-de-France</region><settlement type="city">Versailles</settlement></placeName></affiliation></author><author><name sortKey="Bain, Amanda L" sort="Bain, Amanda L" uniqKey="Bain A" first="Amanda L" last="Bain">Amanda L. Bain</name><affiliation wicri:level="3"><nlm:affiliation>*Signal Transduction Laboratory, Translational Leukaemia Research, QIMR Berghofer Medical Research Institute, Herston, Queensland, Australia; University of Queensland, Brisbane, Queensland, Australia; Institut National De La Recherche Agronomique, Institut Jean-Pierre Bourgin, Unité Mixte de Recherche 1318, Équipes de Recherche Labellisées Centre National de la Recherche Scientifique 3559, Saclay Plant Sciences, Versailles, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>*Signal Transduction Laboratory, Translational Leukaemia Research, QIMR Berghofer Medical Research Institute, Herston, Queensland, Australia; University of Queensland, Brisbane, Queensland, Australia; Institut National De La Recherche Agronomique, Institut Jean-Pierre Bourgin, Unité Mixte de Recherche 1318, Équipes de Recherche Labellisées Centre National de la Recherche Scientifique 3559, Saclay Plant Sciences, Versailles</wicri:regionArea><placeName><region type="region">Île-de-France</region><region type="old region">Île-de-France</region><settlement type="city">Versailles</settlement></placeName></affiliation></author><author><name sortKey="Tagliaro Jahns, Marina" sort="Tagliaro Jahns, Marina" uniqKey="Tagliaro Jahns M" first="Marina" last="Tagliaro-Jahns">Marina Tagliaro-Jahns</name><affiliation wicri:level="3"><nlm:affiliation>*Signal Transduction Laboratory, Translational Leukaemia Research, QIMR Berghofer Medical Research Institute, Herston, Queensland, Australia; University of Queensland, Brisbane, Queensland, Australia; Institut National De La Recherche Agronomique, Institut Jean-Pierre Bourgin, Unité Mixte de Recherche 1318, Équipes de Recherche Labellisées Centre National de la Recherche Scientifique 3559, Saclay Plant Sciences, Versailles, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>*Signal Transduction Laboratory, Translational Leukaemia Research, QIMR Berghofer Medical Research Institute, Herston, Queensland, Australia; University of Queensland, Brisbane, Queensland, Australia; Institut National De La Recherche Agronomique, Institut Jean-Pierre Bourgin, Unité Mixte de Recherche 1318, Équipes de Recherche Labellisées Centre National de la Recherche Scientifique 3559, Saclay Plant Sciences, Versailles</wicri:regionArea><placeName><region type="region">Île-de-France</region><region type="old region">Île-de-France</region><settlement type="city">Versailles</settlement></placeName></affiliation></author><author><name sortKey="Shi, Wei" sort="Shi, Wei" uniqKey="Shi W" first="Wei" last="Shi">Wei Shi</name><affiliation wicri:level="3"><nlm:affiliation>*Signal Transduction Laboratory, Translational Leukaemia Research, QIMR Berghofer Medical Research Institute, Herston, Queensland, Australia; University of Queensland, Brisbane, Queensland, Australia; Institut National De La Recherche Agronomique, Institut Jean-Pierre Bourgin, Unité Mixte de Recherche 1318, Équipes de Recherche Labellisées Centre National de la Recherche Scientifique 3559, Saclay Plant Sciences, Versailles, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>*Signal Transduction Laboratory, Translational Leukaemia Research, QIMR Berghofer Medical Research Institute, Herston, Queensland, Australia; University of Queensland, Brisbane, Queensland, Australia; Institut National De La Recherche Agronomique, Institut Jean-Pierre Bourgin, Unité Mixte de Recherche 1318, Équipes de Recherche Labellisées Centre National de la Recherche Scientifique 3559, Saclay Plant Sciences, Versailles</wicri:regionArea><placeName><region type="region">Île-de-France</region><region type="old region">Île-de-France</region><settlement type="city">Versailles</settlement></placeName></affiliation></author><author><name sortKey="Lane, Steven W" sort="Lane, Steven W" uniqKey="Lane S" first="Steven W" last="Lane">Steven W. Lane</name><affiliation wicri:level="3"><nlm:affiliation>*Signal Transduction Laboratory, Translational Leukaemia Research, QIMR Berghofer Medical Research Institute, Herston, Queensland, Australia; University of Queensland, Brisbane, Queensland, Australia; Institut National De La Recherche Agronomique, Institut Jean-Pierre Bourgin, Unité Mixte de Recherche 1318, Équipes de Recherche Labellisées Centre National de la Recherche Scientifique 3559, Saclay Plant Sciences, Versailles, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>*Signal Transduction Laboratory, Translational Leukaemia Research, QIMR Berghofer Medical Research Institute, Herston, Queensland, Australia; University of Queensland, Brisbane, Queensland, Australia; Institut National De La Recherche Agronomique, Institut Jean-Pierre Bourgin, Unité Mixte de Recherche 1318, Équipes de Recherche Labellisées Centre National de la Recherche Scientifique 3559, Saclay Plant Sciences, Versailles</wicri:regionArea><placeName><region type="region">Île-de-France</region><region type="old region">Île-de-France</region><settlement type="city">Versailles</settlement></placeName></affiliation></author><author><name sortKey="Khanna, Kum Kum" sort="Khanna, Kum Kum" uniqKey="Khanna K" first="Kum Kum" last="Khanna">Kum Kum Khanna</name><affiliation wicri:level="1"><nlm:affiliation>*Signal Transduction Laboratory, Translational Leukaemia Research, QIMR Berghofer Medical Research Institute, Herston, Queensland, Australia; University of Queensland, Brisbane, Queensland, Australia; Institut National De La Recherche Agronomique, Institut Jean-Pierre Bourgin, Unité Mixte de Recherche 1318, Équipes de Recherche Labellisées Centre National de la Recherche Scientifique 3559, Saclay Plant Sciences, Versailles, France kumkum.khanna@qimrberghofer.edu.au.</nlm:affiliation><country wicri:rule="url">Australie</country><wicri:regionArea>*Signal Transduction Laboratory, Translational Leukaemia Research, QIMR Berghofer Medical Research Institute, Herston, Queensland, Australia; University of Queensland, Brisbane, Queensland, Australia; Institut National De La Recherche Agronomique, Institut Jean-Pierre Bourgin, Unité Mixte de Recherche 1318, Équipes de Recherche Labellisées Centre National de la Recherche Scientifique 3559, Saclay Plant Sciences, Versailles</wicri:regionArea><wicri:noRegion>Versailles</wicri:noRegion></affiliation></author></analytic><series><title level="j">FASEB journal : official publication of the Federation of American Societies for Experimental Biology</title><idno type="eISSN">1530-6860</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Carrier Proteins (metabolism)</term><term>DNA Repair (genetics)</term><term>DNA-Binding Proteins (metabolism)</term><term>Fertility (genetics)</term><term>Fibroblasts (metabolism)</term><term>Fibroblasts (physiology)</term><term>Male</term><term>Mice</term><term>Mice, Inbred C57BL</term><term>Mice, Knockout</term><term>Protein Binding (genetics)</term><term>Spleen (metabolism)</term><term>Spleen (physiology)</term><term>Thymus Gland (metabolism)</term><term>Thymus Gland (physiology)</term><term>Up-Regulation (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Fibroblastes (métabolisme)</term><term>Fibroblastes (physiologie)</term><term>Fécondité (génétique)</term><term>Liaison aux protéines (génétique)</term><term>Mâle</term><term>Protéines de liaison à l'ADN (métabolisme)</term><term>Protéines de transport (métabolisme)</term><term>Rate (métabolisme)</term><term>Rate (physiologie)</term><term>Régulation positive (génétique)</term><term>Réparation de l'ADN (génétique)</term><term>Souris</term><term>Souris de lignée C57BL</term><term>Souris knockout</term><term>Thymus (glande) (métabolisme)</term><term>Thymus (glande) (physiologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Carrier Proteins</term><term>DNA-Binding Proteins</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>DNA Repair</term><term>Fertility</term><term>Protein Binding</term><term>Up-Regulation</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Fécondité</term><term>Liaison aux protéines</term><term>Régulation positive</term><term>Réparation de l'ADN</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Fibroblasts</term><term>Spleen</term><term>Thymus Gland</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Fibroblastes</term><term>Protéines de liaison à l'ADN</term><term>Protéines de transport</term><term>Rate</term><term>Thymus (glande)</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Fibroblastes</term><term>Rate</term><term>Thymus (glande)</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Fibroblasts</term><term>Spleen</term><term>Thymus Gland</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Male</term><term>Mice</term><term>Mice, Inbred C57BL</term><term>Mice, Knockout</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Mâle</term><term>Souris</term><term>Souris de lignée C57BL</term><term>Souris knockout</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">SSB1 and SSB2 are newly identified single-stranded (ss) DNA binding proteins that play a crucial role in genome maintenance in humans. We recently generated a knockout mouse model of Ssb1 and revealed its essential role for neonatal survival. Notably, we found compensatory up-regulation of Ssb2 protein levels in multiple tissues of conditional Ssb1(-/-) mice, suggesting functional compensation between these 2 proteins. We report here the first description of Ssb2(-/-) knockout mice. Surprisingly, unlike Ssb1 knockout mice, Ssb2(-/-) mice are viable and fertile and do not exhibit marked phenotypic changes when compared with their Ssb2(+/+) and Ssb2(+/-) littermates. Notably, we did not detect any pathologic changes in the thymus, spleen, or testes, tissues with the most abundant expression of Ssb2. Moreover, Ssb2(-/-) mouse embryonic fibroblasts (MEFs) did not show any sensitivity to DNA-damaging agents, or defects in DNA repair capacity. However, we observed modest up-regulation of Ssb1 levels in Ssb2(-/-) MEFs as well as in Ssb2(-/-) thymus and spleen, suggesting that Ssb1 is likely able to compensate for the loss of Ssb2 in mice. Altogether, our results show that Ssb2 is dispensable for embryogenesis and adult tissue homeostasis, including thymopoiesis, splenic development, male fertility, and DNA repair in mice.</div></front></TEI><affiliations><list><country><li>Australie</li><li>France</li></country><region><li>Île-de-France</li></region><settlement><li>Versailles</li></settlement></list><tree><country name="France"><region name="Île-de-France"><name sortKey="Boucher, Didier" sort="Boucher, Didier" uniqKey="Boucher D" first="Didier" last="Boucher">Didier Boucher</name></region><name sortKey="Bain, Amanda L" sort="Bain, Amanda L" uniqKey="Bain A" first="Amanda L" last="Bain">Amanda L. Bain</name><name sortKey="Lane, Steven W" sort="Lane, Steven W" uniqKey="Lane S" first="Steven W" last="Lane">Steven W. Lane</name><name sortKey="Shi, Wei" sort="Shi, Wei" uniqKey="Shi W" first="Wei" last="Shi">Wei Shi</name><name sortKey="Tagliaro Jahns, Marina" sort="Tagliaro Jahns, Marina" uniqKey="Tagliaro Jahns M" first="Marina" last="Tagliaro-Jahns">Marina Tagliaro-Jahns</name><name sortKey="Vu, Therese" sort="Vu, Therese" uniqKey="Vu T" first="Therese" last="Vu">Therese Vu</name></country><country name="Australie"><noRegion><name sortKey="Khanna, Kum Kum" sort="Khanna, Kum Kum" uniqKey="Khanna K" first="Kum Kum" last="Khanna">Kum Kum Khanna</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Asie/explor/AustralieFrV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002811 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 002811 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Asie
|area= AustralieFrV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:25917330
|texte= Ssb2/Nabp1 is dispensable for thymic maturation, male fertility, and DNA repair in mice.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:25917330" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a AustralieFrV1
| This area was generated with Dilib version V0.6.33. |  |